Methods

To uncover the precise regional, cellular and subcellular localization of distinct proteins, we use three complementary methods of immunohistochemistry.

Our research group is actively involved in technological innovations in Superresolution Microscopy and Pharmacology.

We use several cell culture preparations and express various constructs in these cells to study the operation and function of endocannabinoid-mediated or endo-cannabinoid-related signaling pathways.

Our key research focus centers on the function of distinct molecular players of the endocannabinoid system and other lipid signaling pathways in the nervous system.

To investigate the functional significance of the signaling pathways, which we have uncovered in our molecular biology and anatomy experiments, we perform whole-cell patch-clamp recordings in acute brain slice preparations.

To label specific neuronal populations or to regulate gene expression levels in given cell types (either by overexpression or by siRNA silencing), in utero electroporation is an emerging approach.